Mouse Anti-GPC3 Recombinant Antibody (clone GC33) (CAT#: HPAB-0561-CN)
An anti-glypican 3 antibody GC33 which has strong GPC3 binding activity. GC33 also exhibits particularly strong CDC activity.
Specific Inquiry
WB
Figure 1 Anti-GPC3 Antibody (HPAB-0561-CN) in WB
Western blot analysis was performed with 0.05 µg (lane 1) and 0/1 µg (lane 2) recombinant human GPC3 protein onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least 1 hour. Membranes were probed with GPC3 Antibody (HPAB-0561-CN) at a dilution of 1:1000 overnight at 4°C on a rocking platform. Then probed with Goat anti-Human IgG HRP secondary antibody at a dilution of 1:8,000 for one hour. Chemiluminescent was detected.
ELISA
Figure 2 Anti-GPC3 Antibody (HPAB-0561-CN) in ELISA
ELISA analysis of HPAB-0561-CN was performed by coating with recombinant human GPC3 antigen. Then blocked with BSA, and incubated with GPC3 Antibody (HPAB-0561-CN) at a starting concentration of 2.5 µg/mL. The HRP-conjugated goat anti-human IgG was used as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Specifications
- Immunogen
- A fusion protein containing a peptide from 524 Ala to 563 Lys of GPC3 and GST
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human GPC3
- Species Reactivity
- Human
- Clone
- GC33
- Applications
- ELISA
- Related Disease
- Cancer
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- Glypican 3; Intestinal Protein OCI-5; Glypican Proteoglycan 3; GTR2-2; MXR7; Heparan Sulphate Proteoglycan; Secreted Glypican-3;
OCI-5; SGBS1; DGSX; SGBS; SDYS; OCI5; SGB
- Gene ID
- 2719
- UniProt ID
- P51654
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| NABG-069 | Recombinant Anti-Mouse Gpc3 VHH Single Domain Antibody | WB, ELISA, IHC, FuncS | Llama VHH |
| TAB-416CQ | Anti-Human GPC3 Single Domain Antibody (TAB-416CQ), Research Grade Biosimilar | IP, IHC, FuncS, WB, ELISA, Inhib | Single domain antibody |
Customer Reviews and Q&As
Customer Review
Ken***h
Excellent for ELISA
Excellent for ELISA
In my pursuit of improving hepatocellular carcinoma (HCC) diagnostic methods, the Mouse Anti - GPC3 Recombinant Antibody (clone GC33) has been a game - changer. When testing serum samples from HCC patients in ELISA, I incubated the antibody at 37°C for an hour. It effectively detected GPC3, producing clear and reproducible results, which is invaluable for my research.
15-Jan-23
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# HPAB-0561-CN, RRID: AB_3111400)
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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