Mouse Anti-GPC3 Recombinant Antibody (clone GC33)

Name: Mouse Anti-GPC3 Recombinant Antibody (clone GC33)
SKU: HPAB-0561-CN

CAT#: HPAB-0561-CN

An anti-glypican 3 antibody GC33 which has strong GPC3 binding activity. GC33 also exhibits particularly strong CDC activity.

Tested Data Gene Expression

Figure 1 Anti-GPC3 Antibody (HPAB-0561-CN) in WB

Western blot analysis was performed with 0.05 µg (lane 1) and 0/1 µg (lane 2) recombinant human GPC3 protein onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least 1 hour. Membranes were probed with GPC3 Antibody (HPAB-0561-CN) at a dilution of 1:1000 overnight at 4°C on a rocking platform. Then probed with Goat anti-Human IgG HRP secondary antibody at a dilution of 1:8,000 for one hour. Chemiluminescent was detected.

ELISA

Figure 2 Anti-GPC3 Antibody (HPAB-0561-CN) in ELISA

ELISA analysis of HPAB-0561-CN was performed by coating with recombinant human GPC3 antigen. Then blocked with BSA, and incubated with GPC3 Antibody (HPAB-0561-CN) at a starting concentration of 2.5 µg/mL. The HRP-conjugated goat anti-human IgG was used as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.

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Subcellular Location Figure 1 IF staining of human cell line Hep G2

Immunofluorescent staining of human cell line Hep G2 shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/6316/1404_H7_2_selected.jpg

RNA Expression Figure 2 RNA cell line category: Group enriched (HAP1, Hep G2)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000147257-GPC3

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Specifications

Immunogen
A fusion protein containing a peptide from 524 Ala to 563 Lys of GPC3 and GST

Host Species
Mouse

Type
Mouse IgG

Specificity
Human GPC3

Species Reactivity
Human

Clone
GC33

Applications
ELISA

Related Disease
Cancer

Product Property

Purity
>95% as determined by SDS-PAGE and HPLC analysis

Concentration
Please refer to the vial label for the specific concentration.

Buffer
PBS

Preservative
No preservatives

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Alternative Names
Glypican 3; Intestinal Protein OCI-5; Glypican Proteoglycan 3; GTR2-2; MXR7; Heparan Sulphate Proteoglycan; Secreted Glypican-3;
OCI-5; SGBS1; DGSX; SGBS; SDYS; OCI5; SGB

Gene ID
2719

UniProt ID
P51654

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Breast cancer biomarkers at key points during disease progression

Ken***h
15.Jan,23

Excellent for ELISA

In my pursuit of improving hepatocellular carcinoma (HCC) diagnostic methods, the Mouse Anti - GPC3 Recombinant Antibody (clone GC33) has been a game - changer. When testing serum samples from HCC patients in ELISA, I incubated the antibody at 37°C for an hour. It effectively detected GPC3, producing clear and reproducible results, which is invaluable for my research.

Cite This Product

To accurately reference this product in your publication, please use the following citation information:

(Creative Biolabs Cat# HPAB-0561-CN, RRID: AB_3111400)

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Downloadable Resources

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - HPAB-0561-CN.pdf

MSDS

MSDS - HPAB-0561-CN.pdf

COA

Certificate of Analysis Lookup

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